Journal: Bio-protocol
Article Title: Single-Particle Tracking of AMPA Receptor-Containing Vesicles
doi: 10.21769/BioProtoc.5325
Figure Lengend Snippet: SLIMfast is an in-house MATLAB program based on the multiple-target tracing (MTT) algorithm developed by Serge et al. [20] for single-particle tracking. Arrows , cycle through the frames of the timelapse sequence; PLAY MOV , play the timelapse sequence; OPT , opens a graphical user interface (GUI) with options for acquisition, localization, and tracking parameters; MAX PROJ , creates a figure with a maximum intensity projection of the timelapse sequence; AVE PROJ , creates a figure with an average intensity projection of the timelapse sequence; TEST LOC , locates the particles on the current frame of the timelapse sequence; LOC ALL , performs localization on all particles in the timelapse sequence; GEN TRAJ , performs reconnection to generate trajectories.
Article Snippet: Use the command run SLIMfast in the MATLAB command window, which will open a graphical user interface (GUI) for SLIMfast , a program that uses a multiple-target tracing (MTT) algorithm to achieve 2D single-molecule localization (x,y) [20].
Techniques: Single-particle Tracking, Sequencing